This page (under endless and slow construction) will contain protocols that we commonly use. We also list some links to useful protocols compiled by others.

Introductary Cell Biology Protocols:

• Cell Biology Laboratory Manual - pitched to undergraduates, this site contains some useful basics like a section on statistics, calculating units, and using a microscope.

GPCR-specific protocols:

• Regulation of G-Protein Coupled Receptor Function and Expression. Jeffrey Benovic, ed. Wiley-Liss, 2000.
  Read a summary from Amazon.com

Immunofluorescence

• designing and generating anti-peptide antibodies guide from PE biosystems
• permeabilized staining of tagged receptors in fixed cells
• "feeding" antibody against tagged receptors to live cells in order to label plasma membrane receptors
• the Mitchison lab has great protocols on generating and using antibodies, particularly for visualizing the cytoskeleton.
• Adobe Photoshop Protocols

Tissue Culture

• our own protocol for transfection of adherent cells
• Donis-Keller lab protocols on culturing human cells

Tissue Culture

• Donis-Keller lab protocols on culturing human cells
• our own protocol for transfection of adherent cells

Receptor Biochemistry

• measuring total cell surface receptor by biotinylation
• measuring receptor internalization by flow cytometry
• Bart's protocols - immunoprecipitation

Molecular Biology

• Epitope Tagging, Basic Laboratory Methods. PDF manual from Roche website.

General lab manuals and equipment care

  •  pH meter Care and Troubleshooting (Barron et al review)- pHmetercareandfeeding.pdf
  •  Thermocycler (GeneAmp2400) maintenance (GeneAmp2400maintenance.pdf), coolant instructions (GeneAmp2400coolant.pdf)